ASAH1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-ASAH1 full length
Host
Human
Theoretical MW (kDa)
45
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ASAH1 antibody (H00000427-M01 ) by Western Blots.
SDS-PAGE Gel
ASAH1 transfected lysate
Western Blot
Lane 1: ASAH1 transfected lysate ( 45 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — ASAH1
Entrez GeneID
427GeneBank Accession#
NM_177924Protein Accession#
NP_808592Gene Name
ASAH1
Gene Alias
AC, ASAH, FLJ21558, FLJ22079, PHP, PHP32
Gene Description
N-acylsphingosine amidohydrolase (acid ceramidase) 1
Omim ID
228000Gene Ontology
HyperlinkGene Summary
This gene encodes a heterodimeric protein consisting of a nonglycosylated alpha subunit and a glycosylated beta subunit that is cleaved to the mature enzyme posttranslationally. The encoded protein catalyzes the synthesis and degradation of ceramide into sphingosine and fatty acid. Mutations in this gene have been associated with a lysosomal storage disorder known as Farber disease. Multiple transcript variants encoding several distinct isoforms have been identified for this gene. [provided by RefSeq
Other Designations
N-acylsphingosine amidohydrolase 1|OTTHUMP00000122482|acylsphingosine deacylase|putative 32 kDa heart protein
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Interactome
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Disease
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