LEF1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)
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Specification
Transfected Cell Line
HEK293
Plasmid
pCMV-LEF1 full length
Host
Human
Theoretical MW (kDa)
44
Lysis Buffer
Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
Concentration
2 mg/ml
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-LEF1 antibody (H00051176-M05) by Western Blots.
SDS-PAGE Gel
LEF1 transfected lysate
Western Blot
Lane 1: LEF1 transfected lysate ( 44 KDa).
Lane 2: Non-transfected lysate.Recommend Usage
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Storage Buffer
In modified RIPA Lysis Buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
Immunoprecipitation
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Gene Info — LEF1
Entrez GeneID
51176GeneBank Accession#
BC050632Protein Accession#
AAH50632Gene Name
LEF1
Gene Alias
DKFZp586H0919, TCF1ALPHA
Gene Description
lymphoid enhancer-binding factor 1
Omim ID
153245Gene Ontology
HyperlinkGene Summary
LEF1 is a nuclear protein that is expressed in pre-B and T cells. It binds to a functionally important site in the T-cell receptor-alpha (TCRA; MIM 186880) enhancer and confers maximal enhancer activity. LEF1 belongs to a family of regulatory proteins that share homology with high mobility group protein-1 (HMG1; MIM 163905) (Waterman et al., 1991 [PubMed 2010090]; van Genderen et al., 1994 [PubMed 7958926]).[supplied by OMIM
Other Designations
lymphoid enhancer binding factor-1
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Interactome
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Pathway
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Disease
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