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CSTF2 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L065T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CSTF2 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 61
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CSTF2 antibody (H00001478-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L065T6
    CSTF2 transfected lysate
    Western Blot
    QC Testing of L065T6
    Lane 1: CSTF2 transfected lysate ( 61 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1478
  • Gene Name:
  • CSTF2
  • Gene Alias:
  • CstF-64
  • Gene Description:
  • cleavage stimulation factor, 3' pre-RNA, subunit 2, 64kDa
  • Gene Summary:
  • This gene encodes a nuclear protein with an RRM (RNA recognition motif) domain. The protein is a member of the cleavage stimulation factor (CSTF) complex that is involved in the 3' end cleavage and polyadenylation of pre-mRNAs. Specifically, this protein binds GU-rich elements within the 3'-untranslated region of mRNAs. [provided by RefSeq
  • Other Designations:
  • CF-1 64 kDa subunit,OTTHUMP00000024310,betaCstF-64 variant 2,cleavage stimulation factor subunit 2,cleavage stimulation factor, 64kDa subunit
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