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BID HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L056T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-BID full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 22
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-BID antibody (H00000637-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L056T6
    BID transfected lysate
    Western Blot
    QC Testing of L056T6
    Lane 1: BID transfected lysate ( 22 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 637
  • Gene Name:
  • BID
  • Gene Alias:
  • FP497,MGC15319,MGC42355
  • Gene Description:
  • BH3 interacting domain death agonist
  • Gene Summary:
  • This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq
  • Other Designations:
  • BH3-interacting domain death agonist,BID isoform ES(1b),BID isoform L(2),BID isoform Si6,Human BID coding sequence,OTTHUMP00000196197,apoptic death agonist,desmocollin type 4
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