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CTH HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L054T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CTH full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 45
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CTH antibody (H00001491-M03) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L054T6
    CTH transfected lysate
    Western Blot
    QC Testing of L054T6
    Lane 1: CTH transfected lysate ( 45 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1491
  • Gene Name:
  • CTH
  • Gene Alias:
  • MGC9471
  • Gene Description:
  • cystathionase (cystathionine gamma-lyase)
  • Gene Summary:
  • This gene encodes a cytoplasmic enzyme in the trans-sulfuration pathway that converts cystathione derived from methionine into cysteine. Glutathione synthesis in the liver is dependent upon the availability of cysteine. Mutations in this gene cause cystathioninuria. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • OTTHUMP00000010945,OTTHUMP00000010946,cystathionase,cysteine desulfhydrase,gamma-cystathionase,homoserine deaminase,homoserine dehydratase
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