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CREB1 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)   

  • Catalog # : L048T6
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  • 100 ug
  • USD $ 239
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CREB1 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 37
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 1.9 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CREB1 antibody (H00001385-M02) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L048T6
    CREB1 transfected lysate
    Western Blot
    QC Testing of L048T6
    Lane 1: CREB1 transfected lysate ( 37 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 1385
  • Gene Name:
  • CREB1
  • Gene Alias:
  • CREB,MGC9284
  • Gene Description:
  • cAMP responsive element binding protein 1
  • Gene Summary:
  • This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq
  • Other Designations:
  • active transcription factor CREB,cAMP-response element-binding protein-1,transactivator protein
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