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CCL13 HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L044T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-CCL13 full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 11
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-CCL13 antibody () by Western Blots.
    SDS-PAGE Gel
    QC Testing of L044T6
    CCL13 transfected lysate
    Western Blot
    QC Testing of L044T6
    Lane 1: CCL13 transfected lysate ( 11 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 6357
  • Gene Name:
  • CCL13
  • Gene Alias:
  • CKb10,MCP-4,MGC17134,NCC-1,NCC1,SCYA13,SCYL1
  • Gene Description:
  • chemokine (C-C motif) ligand 13
  • Gene Summary:
  • This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq
  • Other Designations:
  • CK-beta-10,monocyte chemoattractant protein 4,monocyte chemotactic protein 4,new CC chemokine 1,small inducible cytokine A13,small inducible cytokine subfamily A (Cys-Cys), member 13
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