Transient overexpression cell lysate was tested with Anti-CCL13 antibody () by Western Blots. SDS-PAGE Gel
CCL13 transfected lysate Western Blot
Lane 1: CCL13 transfected lysate ( 11 KDa). Lane 2: Non-transfected lysate.
Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
In modified RIPA Lysis Buffer.
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
This gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq
CK-beta-10,monocyte chemoattractant protein 4,monocyte chemotactic protein 4,new CC chemokine 1,small inducible cytokine A13,small inducible cytokine subfamily A (Cys-Cys), member 13