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AXL HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L029T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-AXL full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 98
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-AXL antibody (H00000558-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L029T6
    AXL transfected lysate
    Western Blot
    QC Testing of L029T6
    Lane 1: AXL transfected lysate ( 98 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 558
  • Gene Name:
  • AXL
  • Gene Alias:
  • JTK11,UFO
  • Gene Description:
  • AXL receptor tyrosine kinase
  • Gene Summary:
  • The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, this protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation and can also mediate cell aggregation by homophilic binding. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq
  • Other Designations:
  • AXL transforming sequence/gene,oncogene AXL
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