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Last updated: 2014/12/14
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ACTB HEK293 Cell Transient Overexpression Lysate(Non-Denatured)

  • Catalog # : L015T6
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  • Specification
  • Transfected Cell Line:
  • HEK293
  • Plasmid:
  • pCMV-ACTB full length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 42
  • Lysis Buffer:
  • Modified RIPA Lysis Buffer:50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF.
  • Concentration:
  • 2 mg/ml
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-ACTB antibody (H00000060-M01) by Western Blots.
    SDS-PAGE Gel
    QC Testing of L015T6
    ACTB transfected lysate
    Western Blot
    QC Testing of L015T6
    Lane 1: ACTB transfected lysate ( 42 KDa).
    Lane 2: Non-transfected lysate.
  • Recommend Usage:
  • Use it directly for immuno-precipitation, or heat lysate with SDS gel loading buffer to 95°C for 5 minutes followed by rapid cooling for western blot application. If dissociating conditions are required, add reducing agent prior to heating.
  • Storage Buffer:
  • In modified RIPA Lysis Buffer.
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Immunoprecipitation
  • Gene Information
  • Entrez GeneID:
  • 60
  • Gene Name:
  • ACTB
  • Gene Alias:
  • PS1TP5BP1
  • Gene Description:
  • actin, beta
  • Gene Summary:
  • This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. [provided by RefSeq
  • Other Designations:
  • PS1TP5-binding protein 1,actin, cytoplasmic 1,beta actin,beta cytoskeletal actin
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