VEGFA (Human) ELISA Kit
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Specification
Product Description
VEGFA (Human) ELISA Kit is intended for the quantitative measurement of human VEGFA.
Suitable Sample
Body Fluid, Cell Culture Supernatant, Plasma, Serum, Tissue Lysate
Sample Volume
100 uL
Label
HRP-conjugated
Detection Method
Colorimetric
Assay Type
Quantitative
Calibration Range
31.2 to 2000 pg/mL (Serum, Plasema, Body Fluid)
15.6 to 1000 pg/mL (Cell Culture Supernate)Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard Curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store at 4°C for 4 months, or at -20°C for 8 months.
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Applications
Quantification
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Gene Info — VEGFA
Entrez GeneID
7422Gene Name
VEGFA
Gene Alias
MGC70609, VEGF, VEGF-A, VPF
Gene Description
vascular endothelial growth factor A
Gene Ontology
HyperlinkGene Summary
This gene is a member of the PDGF/VEGF growth factor family and encodes a protein that is often found as a disulfide linked homodimer. This protein is a glycosylated mitogen that specifically acts on endothelial cells and has various effects, including mediating increased vascular permeability, inducing angiogenesis, vasculogenesis and endothelial cell growth, promoting cell migration, and inhibiting apoptosis. Elevated levels of this protein is linked to POEMS syndrome, also known as Crow-Fukase syndrome. Mutations in this gene have been associated with proliferative and nonproliferative diabetic retinopathy. Alternatively spliced transcript variants, encoding either freely secreted or cell-associated isoforms, have been characterized. There is also evidence for the use of non-AUG (CUG) translation initiation sites upstream of, and in-frame with the first AUG, leading to additional isoforms. [provided by RefSeq
Other Designations
vascular endothelial growth factor isoform VEGF165|vascular permeability factor
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Interactome
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Pathway
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Disease
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Publication Reference
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SA/G hydrogel containing NRF2-engineered HEK-293-derived CM improves wound healing efficacy of WJ-MSCs in a rat model of excision injury.
Reza Sabzevari, Amaneh Mohammadi Roushandeh, Zahra Alijani-Ghazyani, Ali Jahanian-Najafabadi, Mehryar Habibi Roudkenar.
Journal of Tissue Viability 2021 Nov; 30(4):527.
Application:Quant, Human, HEK 293 cells.
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Conditioned Medium Harvested from Hif1α Engineered Mesenchymal Stem cells Ameliorates LAD-Occlusion -Induced Injury in Rat Acute Myocardial Ischemia Model.
Zahra Alijani-Ghazyani, Amaneh Mohammadi Roushandeh, Reza Sabzevari, Arsalan Salari, Mohammad Taghi Razavi Toosi, Ali Jahanian-Najafabadi, Mehryar Habibi Roudkenar.
The International Journal of Biochemistry & Cell Biology 2021 Jan; 130:105897.
Application:Quant, Human, Human umbilical cord-derived mesenchymal stem cells.
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SA/G hydrogel containing hCAP-18/LL-37-engineered WJ-MSCs-derived conditioned medium promoted wound healing in rat model of excision injury.
Reza Sabzevari, Amaneh Mohammadi Roushandeh, Ahmad Mehdipour, Mauro Alini, Mehryar Habibi Roudkenar.
Life Sciences 2020 Nov; 261:118381.
Application:Quant, Human, Human Wharton's jelly derived-mesenchymal stem cells.
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Resveratrol-delivery vehicle with anti-VEGF activity carried to human retinal pigmented epithelial cells exposed to high-glucose induced conditions.
Rugină D, Ghiman R, Focșan M, Tăbăran F, Copaciu F, Suciu M, Pintea A, Aștilean S.
Colloids and Surfaces. B, Biointerfaces 2019 Sep; 181:66.
Application:ELISA, Human, Culture medium of D407 cells.
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SA/G hydrogel containing NRF2-engineered HEK-293-derived CM improves wound healing efficacy of WJ-MSCs in a rat model of excision injury.
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