One-Step ChIP Kit
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Result Data
Result Data
The enrichment of RNA polymerase II in GAPDH and MLH1 promoters with chromatin extract from formadehyde fixed colone cancer cells.
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Specification
Product Description
One-Step ChIP Kit is use for investigating interactions of proteins and DNA.
Suitable Sample
Chromatin Extract
Sample Volume
The amount of chromatin for each reaction can be 0.1 ug (about 1 x 104cells) to 15 ug (about 1.5 x 106cells). For an optimal reaction, the input chromatin amount should be 5 to 10 ug (about 0.5 to 1 x 106cells), as enrichment of target proteins to genome loci varies and some of the target proteins are of low abundance.
Regulation Status
For research use only (RUO)
Storage Instruction
Store CH1, Non-Immune IgG, Anti-RNA Polymerase II, Proteinase K, GAPDH Primer-Forward, GAPDH Primer-Reverse, and 8-Well Assay Strips (With 1 Frame) at 4°C away from light. Store all other components at room temperature away from light.
Note
Result Data
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Applications
ChIP
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