RBP4 (Human) ELISA Kit
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Specification
Product Description
RBP4 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of human retinol binding protein 4 in serum, plasma, and other biological media.
Suitable Sample
Biological Medium, Plasma, Serum
Sample Volume
100 uL (1:2500 prediluted)
Label
HRP-conjugate
Detection Method
Colorimetric
Assay Type
Quantitative
Intra-Assay
3.03%
Inter-Assay
6.93%
Spiking Recovery
101%
Calibration Range
1.56 to 100 ng/mL
Limit of Detection
1.08 ng/mL
Reactivity
Human
Regulation Status
For research use only (RUO)
Quality Control Testing
Standard curve
The standard curve is for the purpose of illustration only and should not be used to calculate unknowns. A standard curve should be generated each time the assay is performed.
Storage Instruction
Store the kit at 4°C away from light.
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Applications
Quantification
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Gene Info — RBP4
Entrez GeneID
5950Gene Name
RBP4
Gene Alias
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Gene Description
retinol binding protein 4, plasma
Omim ID
180250Gene Ontology
HyperlinkGene Summary
This protein belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. [provided by RefSeq
Other Designations
OTTHUMP00000020114|OTTHUMP00000020116|retinol-binding protein 4, interstitial|retinol-binding protein 4, plasma
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Interactome
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Disease
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Publication Reference
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Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans.
Wang Y, Lam KS, Kraegen EW, Sweeney G, Zhang J, Tso AW, Chow WS, Wat NM, Xu JY, Hoo RL, Xu A.
Clinical Chemistry 2007 Jan; 53(1):34.
Application:Quant, Human, Human serum.
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Serum retinol binding protein 4 contributes to insulin resistance in obesity and type 2 diabetes.
Yang Q, Graham TE, Mody N, Preitner F, Peroni OD, Zabolotny JM, Kotani K, Quadro L, Kahn BB.
Nature 2005 Jul; 436(7049):356.
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Adipocyte/macrophage fatty acid binding proteins control integrated metabolic responses in obesity and diabetes.
Maeda K, Cao H, Kono K, Gorgun CZ, Furuhashi M, Uysal KT, Cao Q, Atsumi G, Malone H, Krishnan B, Minokoshi Y, Kahn BB, Parker RA, Hotamisligil GS.
Cell Metabolism 2005 Feb; 1(2):107.
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Lipocalin-2 is an inflammatory marker closely associated with obesity, insulin resistance, and hyperglycemia in humans.
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