ATP6V1C2 monoclonal antibody (M01), clone 3D5
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More Files
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Western Blot (Cell lysate)
ATP6V1C2 monoclonal antibody (M01), clone 3D5 Western Blot analysis of ATP6V1C2 expression in HeLa ( Cat # L013V1 ).
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATP6V1C2 is approximately 0.03ng/ml as a capture antibody.
Western Blot detection against Immunogen (33 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant ATP6V1C2.
Immunogen
ATP6V1C2 (NP_653184, 188 a.a. ~ 253 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VPKPNYSQWQKTYESLSDMVVPRSTKLITEDKEGGLFTVTLFRKVIEDFKTKAKENKFTVREFYYD
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (82); Rat (83)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
ATP6V1C2 monoclonal antibody (M01), clone 3D5 Western Blot analysis of ATP6V1C2 expression in HeLa ( Cat # L013V1 ).Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged ATP6V1C2 is approximately 0.03ng/ml as a capture antibody.ELISA
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Gene Info — ATP6V1C2
Entrez GeneID
245973GeneBank Accession#
NM_144583Protein Accession#
NP_653184Gene Name
ATP6V1C2
Gene Alias
ATP6C2, VMA5
Gene Description
ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C2
Gene Ontology
HyperlinkGene Summary
This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of eukaryotic intracellular organelles. V-ATPase dependent organelle acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A,three B, and two G subunits, as well as a C, D, E, F, and H subunit. The V1 domain contains the ATP catalytic site. This gene encodes alternate transcriptional splice variants, encoding different V1 domain C subunit isoforms. [provided by RefSeq
Other Designations
ATPase, H+ transporting, lysosomal 42kD, V1 subunit C|OTTHUMP00000115522|V-ATPase C2 subunit|vacuolar H+ ATPase C2
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Interactome
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Pathway
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Disease
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