RDH12 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
RDH12 transfected lysate.
Western Blot
Lane 1: RDH12 transfected lysate ( 34.87 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-RDH12 full-length
Host
Human
Theoretical MW (kDa)
34.87
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-RDH12 antibody (H00145226-B01) by Western Blots.
SDS-PAGE Gel
RDH12 transfected lysate.
Western Blot
Lane 1: RDH12 transfected lysate ( 34.87 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — RDH12
Entrez GeneID
145226GeneBank Accession#
NM_152443.1Protein Accession#
NP_689656.1Gene Name
RDH12
Gene Alias
FLJ30273, LCA3, SDR7C2
Gene Description
retinol dehydrogenase 12 (all-trans/9-cis/11-cis)
Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is an NADPH-dependent retinal reductase whose highest activity is toward 9-cis and all-trans-retinol. The encoded enzyme also plays a role in the metabolism of short-chain aldehydes but does not exhibit steroid dehydrogenase activity. Defects in this gene are a cause of Leber congenital amaurosis type 3 (LCA3). [provided by RefSeq
Other Designations
retinol dehydrogenase 12|retinol dehydrogenase 12, all-trans and 9-cis|short chain dehydrogenase/reductase family 7C, member 2
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Interactome
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Pathway
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Disease
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