Transient overexpression cell lysate was tested with Anti-ELAC2 antibody (H00060528-B01) by Western Blots. SDS-PAGE Gel ELAC2 transfected lysate. Western Blot Lane 1: ELAC2 transfected lysate ( 90.97 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene has a C-terminal domain with tRNA 3′ processing endoribonuclease activity, which catalyzes the removal of the 3' trailer from precursor tRNAs. The protein also interacts with activated Smad family member 2 (Smad2) and its nuclear partner forkhead box H1 (also known as FAST-1), and reduced expression can suppress transforming growth factor-beta induced growth arrest. Mutations in this gene result in an increased risk of prostate cancer. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq
Other Designations:
elaC homolog 2,hereditary prostate cancer,putative prostate cancer susceptibility protein,tRNA Z (long form)