PAK6 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
PAK6 transfected lysate.
Western Blot
Lane 1: PAK6 transfected lysate ( 74.90 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-PAK6 full-length
Host
Human
Theoretical MW (kDa)
74.9
Interspecies Antigen Sequence
Mouse (92); Rat (92)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-PAK6 antibody (H00056924-B01) by Western Blots.
SDS-PAGE Gel
PAK6 transfected lysate.
Western Blot
Lane 1: PAK6 transfected lysate ( 74.90 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — PAK6
Entrez GeneID
56924GeneBank Accession#
NM_020168.3Protein Accession#
NP_064553.1Gene Name
PAK6
Gene Alias
PAK5
Gene Description
p21 protein (Cdc42/Rac)-activated kinase 6
Omim ID
608110Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the p21-activated kinase (PAK) family. The proteins of this family are Rac/Cdc42-associated Ste20-like Ser/Thr protein kinases, characterized by a highly conserved amino-terminal Cdc42/Rac interactive binding (CRIB) domain and a carboxyl-terminal kinase domain. PAK kinases are implicated in the regulation of a number of cellular processes, including cytoskeleton rearrangement, apoptosis and the MAP kinase signaling pathway. The protein encoded by this gene was found to interact with androgen receptor (AR), which is a steroid hormone-dependent transcription factor that is important for male sexual differentiation and development. This gene was found to be highly expressed in testis and prostate tissues and the encoded protein was shown to cotranslocate into the nucleus with AR in response to androgen. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq
Other Designations
p21(CDKN1A)-activated kinase 6|p21-activated kinase 6|p21-activated protein kinase 6
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Interactome
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