VPS35 monoclonal antibody (M02), clone 2D3
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More Files
- More Functions
Western Blot (Tissue lysate)
VPS35 monoclonal antibody (M02), clone 2D3. Western Blot analysis of VPS35 expression in human colon.
Western Blot (Cell lysate)
VPS35 monoclonal antibody (M02), clone 2D3. Western Blot analysis of VPS35 expression in HeLa.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant VPS35.
Immunogen
VPS35 (NP_060676, 697 a.a. ~ 796 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL
Host
Mouse
Reactivity
Human, Rat
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
VPS35 monoclonal antibody (M02), clone 2D3. Western Blot analysis of VPS35 expression in human colon.Western Blot (Cell lysate)
VPS35 monoclonal antibody (M02), clone 2D3. Western Blot analysis of VPS35 expression in HeLa.Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged VPS35 is 0.03 ng/ml as a capture antibody.ELISA
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Gene Info — VPS35
Entrez GeneID
55737GeneBank Accession#
NM_018206Protein Accession#
NP_060676Gene Name
VPS35
Gene Alias
DKFZp434E1211, DKFZp434P1672, FLJ10752, FLJ13588, FLJ20388, MEM3
Gene Description
vacuolar protein sorting 35 homolog (S. cerevisiae)
Omim ID
606931Gene Ontology
HyperlinkGene Summary
This gene belongs to a group of vacuolar protein sorting (VPS) genes. The encoded protein is a component of a large multimeric complex, termed the retromer complex, involved in retrograde transport of proteins from endosomes to the trans-Golgi network. The close structural similarity between the yeast and human proteins that make up this complex suggests a similarity in function. Expression studies in yeast and mammalian cells indicate that this protein interacts directly with VPS35, which serves as the core of the retromer complex. [provided by RefSeq
Other Designations
maternal-embryonic 3|vacuolar protein sorting 35
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Interactome
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Publication Reference
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Endosomal traffic and glutamate synapse activity are increased in VPS35 D620N mutant knock-in mouse neurons, and resistant to LRRK2 kinase inhibition.
Chelsie A Kadgien, Anusha Kamesh, Austen J Milnerwood.
Molecular Brain 2021 Sep; 14(1):143.
Application：IF, IP, WB-Tr, Mouse, Mouse brain, Mouse cortical neurons.
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A new functional role of mitochondria-anchored protein ligase in peroxisome morphology in mammalian cells.
Abhishek Mohanty, Rodolfo Zunino, Vincent Soubannier, Shilpa Dilipkumar.
Journal of Cellular Biochemistry 2021 Nov; 122(11):1686.
Application：WB-Tr, Monkey, COS-7 cells.
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DNAJC13 p.Asn855Ser, implicated in familial parkinsonism, alters membrane dynamics of Sorting Nexin 1.
Follett J, Fox JD, Gustavsson EK, Kadgien C, Munsie LN, Cao LP, Tatarnikov I, Milnerwood AJ, Farrer MJ.
Neuroscience Letters 2019 Jul; 706:114.
Application：IP, Mouse, Mouse cortical neurons.
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Altered dopamine release and monoamine transporters in Vps35 p.D620N knock-in mice.
Cataldi S, Follett J, Fox JD, Tatarnikov I, Kadgien C, Gustavsson EK, Khinda J, Milnerwood AJ, Farrer MJ.
NPJ Parkinson's Disease 2018 Aug; 4:27.
Application：WB-Ti, Mouse, Mouse brains.
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Activity-regulated trafficking of the palmitoyl-acyl transferase DHHC5.
Brigidi GS, Santyr B, Shimell J, Jovellar B, Bamji SX.
Nature Communications 2015 Sep; 6:8200.
Application：IF, Rat, Hippocampal neurons.
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Retromer-dependent neurotransmitter receptor trafficking to synapses is altered by the Parkinson's Disease VPS35 mutation p.D620N.
Munsie LN, Milnerwood AJ, Seibler P, Beccano-Kelly DA, Tatarnikov I, Khinda J, Volta M, Kadgien C, Cao LP, Tapia L, Klein C, Farrer MJ.
Human Molecular Genetics 2015 Mar; 24(6):1691.
Application：IF, WB-Tr, Human, Mouse, Cortex, HEK293 cells.
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A Combined Transgenic Proteomic Analysis and Regulated Trafficking of Neuroligin-2.
Kang Y, Ge Y, Cassidy RM, Lam V, Luo L, Moon KM, Lewis R, Molday RS, Wong RO, Foster LJ, Craig AM.
The Journal of Biological Chemistry 2014 Oct; 289(42):29350.
Application：ICC, Rat, Hippocampal neuron.
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A Novel, Retromer-Independent Role for Sorting Nexins 1 and 2 in RhoG-Dependent Membrane Remodeling.
Prosser DC, Tran D, Schooley A, Wendland B, Ngsee JK.
Traffic (Copenhagen, Denmark) 2010 Oct; 11(10):1347.
Application：IF, WB-Tr, Human, Mouse, CHO, HeLa cells.
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Endosomal traffic and glutamate synapse activity are increased in VPS35 D620N mutant knock-in mouse neurons, and resistant to LRRK2 kinase inhibition.
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