RHOT1 (Human) Recombinant Protein (P01)
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More Files
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Specification
Product Description
Human RHOT1 full-length ORF ( NP_001028738.1, 1 a.a. - 659 a.a.) recombinant protein with GST-tag at N-terminal.
Sequence
MKKDVRILLVGEPRVGKTSLIMSLVSEEFPEEVPPRAEEITIPADVTPERVPTHIVDYSEAEQSDEQLHQEISQANVICIVYAVNNKHSIDKVTSRWIPLINERTDKDSRLPLILVGNKSDLVEYSSMETILPIMNQYTEIETCVECSAKNLKNISELFYYAQKAVLHPTGPLYCPEEKEMKPACIKALTRIFKISDQDNDGTLNDAELNFFQRICFNTPLAPQALEDVKNVVRKHISDGVADSGLTLKGFLFLHTLFIQRGRHETTWTVLRRFGYDDDLDLTPEYLFPLLKIPPDCTTELNHHAYLFLQSTFDKHDLDRDCALSPDELKDLFKVFPYIPWGPDVNNTVCTNERGWITYQGFLSQWTLTTYLDVQRCLEYLGYLGYSILTEQESQASAVTVTRDKKIDLQKKQTQRNVFRCNVIGVKNCGKSGVLQALLGRNLMRQKKIREDHKSYYAINTVYVYGQEKYLLLHDISESEFLTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSISPTDFCRKHKMPPPQAFTCNTADAPSKDIFVKLTTMAMYPHARLRCMCTCNRCTFCICQNFLNSDLLQSVKNKIFTAVLNRHVTQADLKSSTFWLRASFGATVFAVLGFAMYKALLKQR
Host
Wheat Germ (in vitro)
Theoretical MW (kDa)
101.9
Interspecies Antigen Sequence
Mouse (98); Rat (91)
Preparation Method
Purification
Glutathione Sepharose 4 Fast Flow
Quality Control Testing
12.5% SDS-PAGE Stained with Coomassie Blue.
Storage Buffer
50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
Note
Best use within three months from the date of receipt of this protein.
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Applications
Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
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Gene Info — RHOT1
Entrez GeneID
55288GeneBank Accession#
NM_001033566.1Protein Accession#
NP_001028738.1Gene Name
RHOT1
Gene Alias
ARHT1, FLJ11040, FLJ12633, MIRO-1
Gene Description
ras homolog gene family, member T1
Gene Ontology
HyperlinkGene Summary
O
Other Designations
mitochondrial Rho 1|rac-GTP binding protein-like protein
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Interactome
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Disease
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Publication Reference
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A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation.
Li Li, Devon M Conradson, Vinita Bharat, Min Joo Kim, Chung-Han Hsieh, Paras S Minhas, Amanda M Papakyrikos, Aarooran Sivakumaran Durairaj, Anthony Ludlam, Katrin I Andreasson, Linda Partridge, Michael A Cianfrocco and Xinnan Wang.
Nature Metabolism 2021 Sep; 3(9):1241.
Application:Pull-Down, Flies, Fly.
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A mitochondrial membrane-bridging machinery mediates signal transduction of intramitochondrial oxidation.
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