DGCR8 monoclonal antibody (M01), clone 4G3
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More Files
- More Functions
Western Blot (Tissue lysate)
DGCR8 monoclonal antibody (M01), clone 4G3. Western Blot analysis of DGCR8 expression in human spleen.
Western Blot (Transfected lysate)
Western Blot analysis of DGCR8 expression in transfected 293T cell line by DGCR8 monoclonal antibody (M01), clone 4G3.
Lane 1: DGCR8 transfected lysate (Predicted MW: 86 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DGCR8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 6 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DGCR8 is 0.1 ng/ml as a capture antibody.
Western Blot detection against Immunogen (36.41 KDa) .
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Specifications
Product Description
Mouse monoclonal antibody raised against a partial recombinant DGCR8.
Immunogen
DGCR8 (NP_073557, 675 a.a. ~ 771 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.41 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
DGCR8 monoclonal antibody (M01), clone 4G3. Western Blot analysis of DGCR8 expression in human spleen.Western Blot (Transfected lysate)
Western Blot analysis of DGCR8 expression in transfected 293T cell line by DGCR8 monoclonal antibody (M01), clone 4G3.
Lane 1: DGCR8 transfected lysate (Predicted MW: 86 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to DGCR8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 6 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged DGCR8 is 0.1 ng/ml as a capture antibody.ELISA
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Gene Info — DGCR8
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Interactomes
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Diseases
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Publication Reference
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The subcellular topology of the RNAi machinery is multifaceted and reveals adherens junctions as an epithelial hub.
Joyce Nair-Menon., Christina Kingsley., Houda Mesnaoui., Alyssa Risner., Jordan E Jarvis., Peter Lin., Kyrie Wilson., Bärbel Rohrer., Antonis Kourtidis.
Scientific reports 2025 Jul; 15(1):24814.
Application：Immunofluorescence, Western Blot, Human, Caco2 cells, ARPE-19 cells, HUVEC cells, MCF10A cells, HaCaT keratinocytes, HepG2 liver cancer cells, HeLa cells, U2OS cells, HISM cells, colon tissue, liver tissue, pancreas tissue, kidney tissue, bl.
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The subcellular topology of the RNAi machinery ismultifaceted and reveals adherens junctions as anepithelial hub.
Joyce Nair-Menon, Christina Kingsley, Houda Mesnaoui, Peter Lin, Kyrie Wilson, Bärbel Rohrer, Antonis Kourtidis.
Research Square 2025 Feb; 0:0.
Application：IF, Human, Breast immortalized epithelial MCF10A cells, Cervical cancer HeLa cells, HepG2 liver cancer cells, human endothelial HUVEC cells, Immortalized HaCaT keratinocytes, Intestinal HISM fi broblasts, Osteos.
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Predominant Distribution of the RNAi Machinery at Apical Adherens Junctions in Colonic Epithelia Is Disrupted in Cancer.
Nair-Menon J, Daulagala AC, Connor DM, Rutledge L, Penix T, Bridges MC, Wellslager B, Spyropoulos DD, Timmers CD, Broome AM, Kourtidis A.
International Journal of Molecular Sciences 2020 Apr; 21(7):E2559.
Application：IF, Human, Caco2, HT-29, DLD-1, LS174T, HCT116 cells.
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The subcellular topology of the RNAi machinery is multifaceted and reveals adherens junctions as an epithelial hub.
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