KRT20 monoclonal antibody (M01), clone 2G3-1C8
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More Files
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Western Blot (Cell lysate)
KRT20 monoclonal antibody (M01), clone 2G3-1C8 Western Blot analysis of KRT20 expression in A-431 ( Cat # L015V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.
Lane 1: KRT20 transfected lysate(48.5 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell. [antibody concentration 10 ug/ml]
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT20 on U-2 OS cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (72.38 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant KRT20.
Immunogen
KRT20 (AAH31559, 1 a.a. ~ 424 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MDFSRRSFHRSLSSSLQAPVVSTVGMQRLGTTPSVYGGAGGRGIRISNSRHTVNYGSDLTGGGDLFVGNEKMAMQNLNDRLASYLEKVRTLEQSNSKLEVQIKQWYETNAPRAGRDYSAYYRQIEELRSQIKDAQLQNARCVLQIDNAKLAAEDFRLKYETERGIRLTVEADLQGLNKVFDDLTLHKTDLEIQIEELNKDLALLKKEHQEEVDGLHKHLGNTVNVEVDAAPGLNLGVIMNEMRQKYEVMAQKNLQEAKEQFERQTAVLQQQVTVNTEELKGTEVQLTELRRTSQSLEIELQSHLSMKESLEHTLEETKARYSSQLANLQSLLSSLEAQLMQIRSNMERQNNEYHILLDIKTRLEQEIATYRRLLEGEDVKTTEYQLSTLEERDIKKTRKIKTVVQEVVDGKVVSSEVKEVEENI
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (78); Rat (80)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (72.38 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
KRT20 monoclonal antibody (M01), clone 2G3-1C8 Western Blot analysis of KRT20 expression in A-431 ( Cat # L015V1 ).Western Blot (Transfected lysate)
Western Blot analysis of KRT20 expression in transfected 293T cell line by KRT20 monoclonal antibody (M01), clone 2G3-1C8.
Lane 1: KRT20 transfected lysate(48.5 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to KRT20 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged KRT20 is approximately 0.03ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT20 on HeLa cell. [antibody concentration 10 ug/ml]Immunofluorescence
Immunofluorescence of monoclonal antibody to KRT20 on U-2 OS cell. [antibody concentration 10 ug/ml] -
Gene Info — KRT20
Entrez GeneID
54474GeneBank Accession#
BC031559Protein Accession#
AAH31559Gene Name
KRT20
Gene Alias
CD20, CK20, K20, KRT21, MGC35423
Gene Description
keratin 20
Omim ID
608218Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. This cytokeratin is a major cellular protein of mature enterocytes and goblet cells and is specifically expressed in the gastric and intestinal mucosa. The type I cytokeratin genes are clustered in a region of chromosome 17q12-q21. [provided by RefSeq
Other Designations
cytokeratin 20|keratin, type I cytoskeletal 20
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Interactome
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Disease
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