BTBD1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
BTBD1 transfected lysate.
Western Blot
Lane 1: BTBD1 transfected lysate ( 52.8 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-BTBD1 full-length
Host
Human
Theoretical MW (kDa)
52.8
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-BTBD1 antibody (H00053339-B01) by Western Blots.
SDS-PAGE Gel
BTBD1 transfected lysate.
Western Blot
Lane 1: BTBD1 transfected lysate ( 52.8 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — BTBD1
Entrez GeneID
53339GeneBank Accession#
NM_025238.3Protein Accession#
NP_079514.1Gene Name
BTBD1
Gene Alias
C15orf1, NS5ATP8
Gene Description
BTB (POZ) domain containing 1
Omim ID
608530Gene Ontology
HyperlinkGene Summary
The C-terminus of the protein encoded by this gene binds topoisomerase I. The N-terminus contains a proline-rich region and a BTB/POZ domain (broad-complex, Tramtrack and bric a brac/Pox virus and Zinc finger), both of which are typically involved in protein-protein interactions. Subcellularly, the protein localizes to cytoplasmic bodies. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq
Other Designations
BTB domain containing 1|hepatitis C virus NS5A-transactivated protein 8
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