RNF12 purified MaxPab mouse polyclonal antibody (B01P)
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of RNF12 expression in transfected 293T cell line (H00051132-T01) by RNF12 MaxPab polyclonal antibody.
Lane 1: RNF12 transfected lysate(68.64 KDa).
Lane 2: Non-transfected lysate.
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human RNF12 protein.
Immunogen
RNF12 (NP_057204.2, 1 a.a. ~ 624 a.a) full-length human protein.
Sequence
MENSDSNDKGSGDQSAAQRRSQMDRLDREEAFYQFVNNLSEEDYRLMRDNNLLGTPGESTEEELLRRLQQIKEGPPPQNSDENRGGDSSDDVSNGDSIIDWLNSVRQTGNTTRSGQRGNQSWRAVSRTNPNSGDFRFSLEINVNRNNGSQNSENENEPSARRSSGENVENNSQRQVENPRSESTSARPSRSERNSTEALTEVPPTRGQRRARSRSPDHRRTRARAERSRSPLHPMSEIPRRSHHSISSQTFEHPLVNETEGSSRTRHHVTLRQQISGPELLSRGLFAASGTRNASQGAGSSDTAASGESTGSGQRPPTIVLDLQVRRVRPGEYRQRDSIASRTRSRSQTPNNTVTYESERGGFRRTFSRSERAGVRTYVSTIRIPIRRILNTGLSETTSVAIQTMLRQIMTGFGELSYFMYSDSDSEPTGSVSNRNMERAESRSGRGGSGGGSSSGSSSSSSSSSSSSSSSSSSSSPSSSSGGESSETSSDLFEGSNEGSSSSGSSGARREGRHRAPVTFDESGSLPFLSLAQFFLLNEDDDDQPRGLTKEQIDNLAMRSFGENDALKTCSVCITEYTEGNKLRKLPCSHEYHVHCIDRWLSENSTCPICRRAVLASGNRESVV
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (85); Rat (86)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF12 expression in transfected 293T cell line (H00051132-T01) by RNF12 MaxPab polyclonal antibody.
Lane 1: RNF12 transfected lysate(68.64 KDa).
Lane 2: Non-transfected lysate.
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Gene Info — RNF12
Entrez GeneID
51132GeneBank Accession#
NM_016120.2Protein Accession#
NP_057204.2Gene Name
RNF12
Gene Alias
MGC15161, NY-REN-43, RLIM
Gene Description
ring finger protein 12
Omim ID
300379Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a RING-H2 zinc finger protein. It has been shown to be an E3 ubiquitin protein ligase that targets LIM domain binding 1 (LDB1/CLIM), and causes proteasome-dependent degradation of LDB1. This protein and LDB1 are co-repressors of LHX1/LIM-1, a homeodomain transcription factor. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq
Other Designations
LIM domain interacting ring finger protein|OTTHUMP00000023574|OTTHUMP00000023575|ring zinc finger LIM domain binding protein|ring zinc finger protein NY-REN-43antigen
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Interactome
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Publication Reference
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REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.
Gontan C, Mira-Bontenbal H, Magaraki A, Dupont C, Barakat TS, Rentmeester E, Demmers J, Gribnau J.
Nature Communications 2018 Nov; 9(1):4752.
Application：IF, WB-Ce, Mouse, ESCs, Blastocysts.
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Pathogenic variants in E3 ubiquitin ligase RLIM/RNF12 lead to a syndromic X-linked intellectual disability and behavior disorder.
Frints SGM, Ozanturk A, Rodríguez Criado G, Grasshoff U, de Hoon B, Field M, Manouvrier-Hanu S, E Hickey S, Kammoun M, Gripp KW, Bauer C, Schroeder C, Toutain A, Mihalic Mosher T, Kelly BJ, White P, Dufke A, Rentmeester E, Moon S, Koboldt DC, van Roozendaal KEP, Hu H, Haas SA, Ropers HH, Murray L, Haan E, Shaw M, Carroll R, Friend K, Liebelt J, Hobson L, De Rademaeker M, Geraedts J, Fryns JP, Vermeesch J, Raynaud M, Riess O, Gribnau J, Katsanis N, Devriendt K, Bauer P, Gecz J, Golzio C, Gontan C
Molecular Psychiatry 2018 May; [Epub].
Application：WB-Tr, Human, HEK293 cells.
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REX1 is the critical target of RNF12 in imprinted X chromosome inactivation in mice.
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