PIK3R4 monoclonal antibody (M01), clone 1D4
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PIK3R4.
Immunogen
PIK3R4 (NP_055417, 1259 a.a. ~ 1358 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
AGSDMKIRFWDLAYPERSYVVAGSTSSPSVSYYRKIIEGTEVVQEIQNKQKVGPSDDTPRRGPESLPVGHHDIITDVATFQTTQGFIVTASRDGIVKVWK
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (95); Rat (93)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PIK3R4 monoclonal antibody (M01), clone 1D4 Western Blot analysis of PIK3R4 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PIK3R4 expression in transfected 293T cell line by PIK3R4 monoclonal antibody (M01), clone 1D4.
Lane 1: PIK3R4 transfected lysate(153.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PIK3R4 is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PIK3R4 over-expressed 293 cell line, cotransfected with PIK3R4 Validated Chimera RNAi ( Cat # H00030849-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PIK3R4 monoclonal antibody (M01) clone 1D4 (Cat # H00030849-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — PIK3R4
Entrez GeneID
30849GeneBank Accession#
NM_014602Protein Accession#
NP_055417Gene Name
PIK3R4
Gene Alias
MGC102700, VPS15, p150
Gene Description
phosphoinositide-3-kinase, regulatory subunit 4
Omim ID
602610Gene Ontology
HyperlinkGene Summary
O
Other Designations
phosphatidylinositol 3-kinase-associated p150|phosphoinositide-3-kinase, regulatory subunit 4, p150
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Interactome
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Pathway
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