ACF 293T Cell Transient Overexpression Lysate(Denatured)
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-ACF full-length
Host
Human
Theoretical MW (kDa)
65
Interspecies Antigen Sequence
Mouse (90); Rat (91)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-ACF antibody (H00029974-B01P) by Western Blots.
SDS-PAGE Gel
A1CF transfected lysate.
Western Blot
Lane 1: A1CF transfected lysate ( 65.00 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — A1CF
Entrez GeneID
29974GeneBank Accession#
NM_138933Protein Accession#
NP_620311.1Gene Name
A1CF
Gene Alias
ACF, ACF64, ACF65, APOBEC1CF, ASP, MGC163391, RP11-564C4.2
Gene Description
APOBEC1 complementation factor
Gene Ontology
HyperlinkGene Summary
Mammalian apolipoprotein B mRNA undergoes site-specific C to U deamination, which is mediated by a multi-component enzyme complex containing a minimal core composed of APOBEC-1 and a complementation factor encoded by this gene. The gene product has three non-identical RNA recognition motifs and belongs to the hnRNP R family of RNA-binding proteins. It has been proposed that this complementation factor functions as an RNA-binding subunit and docks APOBEC-1 to deaminate the upstream cytidine. Studies suggest that the protein may also be involved in other RNA editing or RNA processing events. Alternative splicing occurs at this locus and three full-length transcript variants, encoding three distinct isoforms, have been described. Additional splicing has been observed but the full-length nature of these variants has not been determined. [provided by RefSeq
Other Designations
APOBEC-1 stimulating protein|OTTHUMP00000019611|OTTHUMP00000019612|OTTHUMP00000019614|OTTHUMP00000019615|OTTHUMP00000061209|apo-B RNA editing protein|apobec-1 complementation factor|apobec-1 complementation factor (ACF) (ASP)
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