GLCE 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
GLCE transfected lysate.
Western Blot
Lane 1: GLCE transfected lysate ( 67.98 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-GLCE full-length
Host
Human
Theoretical MW (kDa)
67.98
Interspecies Antigen Sequence
Mouse (95); Rat (95)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-GLCE antibody (H00026035-B01) by Western Blots.
SDS-PAGE Gel
GLCE transfected lysate.
Western Blot
Lane 1: GLCE transfected lysate ( 67.98 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — GLCE
Entrez GeneID
26035GeneBank Accession#
NM_015554.1Protein Accession#
NP_056369.1Gene Name
GLCE
Gene Alias
HSEPI, KIAA0836
Gene Description
glucuronic acid epimerase
Gene Ontology
HyperlinkGene Summary
Heparan sulfate (HS) is a negatively charged cell surface polysaccharide required for the biologic activities of circulating extracellular ligands. GLCE is responsible for epimerization of D-glucuronic acid (GlcA) to L-iduronic acid (IdoA) of HS, which endows the nascent polysaccharide chain with the ability to bind growth factors and cytokines (Ghiselli and Agrawal, 2005 [PubMed 15853773]).[supplied by OMIM
Other Designations
D-glucuronyl C5-epimerase|UDP-glucuronic acid epimerase|glucuronyl C5-epimerase|heparan sulfate epimerase|heparin/heparan sulfate-glucuronic acid C5-epimerase
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Interactome
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Pathway
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Disease
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