NUP62 monoclonal antibody (M02), clone 2D3
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More Files
- More Functions
Western Blot (Cell lysate)
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot (Cell lysate)
NUP62 monoclonal antibody (M02), clone 2D3 Western Blot analysis of NUP62 expression in Hela S3 NE ( Cat # L013V3 ).
Western Blot (Cell lysate)
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in PC-12(Cat # L012V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of NUP62 expression in transfected 293T cell line by NUP62 monoclonal antibody (M02), clone 2D3.
Lane 1: NUP62 transfected lysate (Predicted MW: 53.3 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NUP62 is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant NUP62.
Immunogen
NUP62 (NP_714941, 423 a.a. ~ 522 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LQHADEEREKTYKLAENIDAQLKRMAQDLKDIIEHLNTSGAPADTSDPLQQICKILNAHMDSLQWIDQNSALLQRKVEEVTKVCEGRRKEQERSFRITFD
Host
Mouse
Reactivity
Human, Mouse, Rat
Interspecies Antigen Sequence
Mouse (79); Rat (79)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
NUP62 monoclonal antibody (M02), clone 2D3 Western Blot analysis of NUP62 expression in Hela S3 NE ( Cat # L013V3 ).Western Blot (Cell lysate)
NUP62 monoclonal antibody (M02), clone 2D3. Western Blot analysis of NUP62 expression in PC-12(Cat # L012V1 ).Western Blot (Transfected lysate)
Western Blot analysis of NUP62 expression in transfected 293T cell line by NUP62 monoclonal antibody (M02), clone 2D3.
Lane 1: NUP62 transfected lysate (Predicted MW: 53.3 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged NUP62 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — NUP62
Entrez GeneID
23636GeneBank Accession#
NM_153719Protein Accession#
NP_714941Gene Name
NUP62
Gene Alias
DKFZp547L134, FLJ20822, FLJ43869, IBSN, MGC841, SNDI, p62
Gene Description
nucleoporin 62kDa
Gene Ontology
HyperlinkGene Summary
The nuclear pore complex is a massive structure that extends across the nuclear envelope, forming a gateway that regulates the flow of macromolecules between the nucleus and the cytoplasm. Nucleoporins are the main components of the nuclear pore complex in eukaryotic cells. The protein encoded by this gene is a member of the FG-repeat containing nucleoporins and is localized to the nuclear pore central plug. This protein associates with the importin alpha/beta complex which is involved in the import of proteins containing nuclear localization signals. Multiple transcript variants of this gene encode a single protein isoform. [provided by RefSeq
Other Designations
nuclear pore glycoprotein p62
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Interactome
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Publication Reference
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Topical Bixin Confers NRF2-Dependent Protection Against Photodamage and Hair Graying in Mouse Skin.
Rojo de la Vega M, Zhang DD, Wondrak GT.
Frontiers in Pharmacology 2018 Mar; 9:287.
Application：WB-Ti, Mouse, Mouse skin.
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Topical Bixin Confers NRF2-Dependent Protection Against Photodamage and Hair Graying in Mouse Skin.
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