MRPS27 MaxPab mouse polyclonal antibody (B01)
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More Files
- More Functions
Western Blot (Tissue lysate)
MRPS27 MaxPab polyclonal antibody. Western Blot analysis of MRPS27 expression in human kidney.
Western Blot (Transfected lysate)
Western Blot analysis of MRPS27 expression in transfected 293T cell line (H00023107-T01) by MRPS27 MaxPab polyclonal antibody.
Lane 1: MRPS27 transfected lysate(18.48 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to MRPS27 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human MRPS27 protein.
Immunogen
MRPS27 (AAH30521, 1 a.a. ~ 168 a.a) full-length human protein.
Sequence
MPLIWKPGYLDRALQVMEKVAASPEDIKLCREALDVLGAVLKALTSADGASEEQSQNDEDNQGSEKLVEQLDIEETEQSKLPQYLERFKALHSKLQALGKIESEGLLSLTTQLVKEKLSTCEAEDIATYEQNLQQWHLDLVQLIQREQQQREQAKQEYQAQKAAKASA
Host
Mouse
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Tissue lysate)
MRPS27 MaxPab polyclonal antibody. Western Blot analysis of MRPS27 expression in human kidney.Western Blot (Transfected lysate)
Western Blot analysis of MRPS27 expression in transfected 293T cell line (H00023107-T01) by MRPS27 MaxPab polyclonal antibody.
Lane 1: MRPS27 transfected lysate(18.48 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to MRPS27 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — MRPS27
Entrez GeneID
23107GeneBank Accession#
BC030521Protein Accession#
AAH30521Gene Name
MRPS27
Gene Alias
FLJ21764, FLJ23348, KIAA0264, MRP-S27, S27mt
Gene Description
mitochondrial ribosomal protein S27
Gene Ontology
HyperlinkGene Summary
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that may be a functional partner of the death associated protein 3 (DAP3). [provided by RefSeq
Other Designations
mitochondrial 28S ribosomal protein S27
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Interactome
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Publication Reference
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hNOA1 interacts with complex I and DAP3 and regulates mitochondrial respiration and apoptosis.
Tang T, Zheng B, Chen SH, Murphy AN, Kudlicka K, Zhou H, Farquhar MG.
The Journal of Biological Chemistry 2008 Dec; 284(8):5414.
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hNOA1 interacts with complex I and DAP3 and regulates mitochondrial respiration and apoptosis.
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