DKK1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
DKK1 transfected lysate.
Western Blot
Lane 1: DKK1 transfected lysate ( 28.7 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-DKK1 full-length
Host
Human
Theoretical MW (kDa)
28.7
Interspecies Antigen Sequence
Mouse (81)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-DKK1antibody (H00022943-D01P)byWesternBlots.
SDS-PAGE Gel
DKK1 transfected lysate.
Western Blot
Lane 1: DKK1 transfected lysate ( 28.7 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — DKK1
Entrez GeneID
22943GeneBank Accession#
NM_012242Protein Accession#
NP_036374.1Gene Name
DKK1
Gene Alias
DKK-1, SK
Gene Description
dickkopf homolog 1 (Xenopus laevis)
Omim ID
605189Gene Ontology
HyperlinkGene Summary
This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma. [provided by RefSeq
Other Designations
OTTHUMP00000019617|dickkopf homolog 1|dickkopf related protein-1|dickkopf-1 like
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Interactome
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Pathway
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Disease
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