MAP4K5 monoclonal antibody (M01), clone 3H3
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant MAP4K5.
Immunogen
MAP4K5 (AAH36013, 361 a.a. ~ 470 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SSDPNFMLQWNPFVDGANTGKSTSKRAIPPPLPPKPRISSYPEDNFPDEEKASTIKHCPDSESRAPQILRRQSSPSCGPVAETSSIGNGDGISKLMSENTEGSAQAPQLP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (96); Rat (96)
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of MAP4K5 expression in transfected 293T cell line by MAP4K5 monoclonal antibody (M01), clone 3H3.
Lane 1: MAP4K5 transfected lysate(95.04 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged MAP4K5 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of MAP4K5 over-expressed 293 cell line, cotransfected with MAP4K5 Validated Chimera RNAi ( Cat # H00011183-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with MAP4K5 monoclonal antibody (M01) clone 3H3 (Cat # H00011183-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — MAP4K5
Entrez GeneID
11183GeneBank Accession#
BC036013Protein Accession#
AAH36013Gene Name
MAP4K5
Gene Alias
GCKR, KHS, KHS1, MAPKKKK5
Gene Description
mitogen-activated protein kinase kinase kinase kinase 5
Omim ID
604923Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the serine/threonine protein kinase family, that is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. This kinase was shown to activate Jun kinase in mammalian cells, which suggested a role in stress response. Two alternatively spliced transcript variants encoding the same protein have been described for this gene. [provided by RefSeq
Other Designations
germinal center kinase-related|kinase homologous to SPS1/STE20
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Interactome
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Disease
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