POLS monoclonal antibody (M01), clone 2F8
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant POLS.
Immunogen
POLS (NP_008930, 2 a.a. ~ 110 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SPCPEEAAMRREVVKRIETVVKDLWPTADVQIFGSFSTGLYLPTSDIDLVVFGKWERPPLQLLEQALRKHNVAEPCSIKVLDKATVPIIKLTDQETEVKVDISFNMETG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (94); Rat (94)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (37.73 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged POLS is approximately 0.3ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of POLS over-expressed 293 cell line, cotransfected with POLS Validated Chimera RNAi ( Cat # H00011044-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with POLS monoclonal antibody (M01), clone 2F8 (Cat # H00011044-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — POLS
Entrez GeneID
11044GeneBank Accession#
NM_006999Protein Accession#
NP_008930Gene Name
POLS
Gene Alias
LAK-1, POLK, TRF4, TRF4-1
Gene Description
polymerase (DNA directed) sigma
Omim ID
605198Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a DNA polymerase that is likely involved in DNA repair. In addition, the encoded protein may be required for sister chromatid adhesion. [provided by RefSeq
Other Designations
DNA polymerase kappa|DNA polymerase sigma|OTTHUMP00000115520|polymerase (DNA-directed) sigma|topoisomerase-related function protein 4-1
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Interactome
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Pathway
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Disease
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