AKT3 monoclonal antibody (M08), clone 6E11
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More Files
- More Functions
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in NIH/3T3 ( Cat # L018V1 ).
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11 Western Blot analysis of AKT3 expression in HeLa ( Cat # L013V1 ).
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in Raw 264.7 ( Cat # L024V1 ).
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in K-562 ( Cat # L009V1 ).
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in PC-12 ( Cat # L012V1 ).
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in MCF-7 ( Cat # L046V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of AKT3 expression in transfected 293T cell line by AKT3 monoclonal antibody (M08), clone 6E11.
Lane 1: AKT3 transfected lysate(55.8 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AKT3 is approximately 0.03ng/ml as a capture antibody.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between PRKCZ and AKT3. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-AKT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Western Blot detection against Immunogen (35.53 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant AKT3.
Immunogen
AKT3 (AAD29089, 100 a.a. ~ 189 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
EAIQAVADRLQRQEEERMNCSPTSQIDNIGEEEMDASTTHHKRKTMNDFDYLKLLGKGTFGKVILVREKASGKYYAMKILKKEVIIAKDE
Host
Mouse
Reactivity
Human, Mouse, Rat
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (35.53 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in NIH/3T3 ( Cat # L018V1 ).Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11 Western Blot analysis of AKT3 expression in HeLa ( Cat # L013V1 ).Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in Raw 264.7 ( Cat # L024V1 ).Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in K-562 ( Cat # L009V1 ).Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in PC-12 ( Cat # L012V1 ).Western Blot (Cell lysate)
AKT3 monoclonal antibody (M08), clone 6E11. Western Blot analysis of AKT3 expression in MCF-7 ( Cat # L046V1 ).Western Blot (Transfected lysate)
Western Blot analysis of AKT3 expression in transfected 293T cell line by AKT3 monoclonal antibody (M08), clone 6E11.
Lane 1: AKT3 transfected lysate(55.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged AKT3 is approximately 0.03ng/ml as a capture antibody.ELISA
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between PRKCZ and AKT3. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-AKT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue). -
Gene Info — AKT3
Entrez GeneID
10000GeneBank Accession#
AF124141Protein Accession#
AAD29089Gene Name
AKT3
Gene Alias
DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2
Gene Description
v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma)
Omim ID
611223Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake. This kinase has been shown to be stimulated by platelet-derived growth factor (PDGF), insulin, and insulin-like growth factor 1 (IGF1). Alternatively splice transcript variants encoding distinct isoforms have been described. [provided by RefSeq
Other Designations
OTTHUMP00000037911|OTTHUMP00000037912|RAC-gamma serine/threonine protein kinase|protein kinase B gamma|serine threonine protein kinase, Akt-3|v-akt murine thymoma viral oncogene homolog 3
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Interactome
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Pathway
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Disease
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