RNF14 MaxPab mouse polyclonal antibody (B01)
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human RNF14 protein.
Immunogen
RNF14 (NP_899645.1, 1 a.a. ~ 348 a.a) full-length human protein.
Sequence
MQFLKEETLAYLNIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSDVDQEEIVDERAVQDVESLSNLIQEILDFDQAQQIKCFNSKLFLCSICFCEKLGSECMYFLECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRLLLQSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYHGVSPCKVTAEKLMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKLDGCNKMTCTGCMQYFCWICMGSLSRANPYKHFNDPGSPCFNRLFYAVDVDDDIWEDEVED
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (88); Rat (88)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of RNF14 expression in transfected 293T cell line (H00009604-T01) by RNF14 MaxPab polyclonal antibody.
Lane 1: RNF14 transfected lysate(38.28 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to RNF14 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — RNF14
Entrez GeneID
9604GeneBank Accession#
NM_183398.1Protein Accession#
NP_899645.1Gene Name
RNF14
Gene Alias
ARA54, FLJ26004, HFB30, HRIHFB2038, TRIAD2
Gene Description
ring finger protein 14
Omim ID
605675Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene contains a RING zinc finger, a motif known to be involved in protein-protein interactions. This protein interacts with androgen receptor (AR) and may function as a coactivator that induces AR target gene expression in prostate. A dominant negative mutant of this gene has been demonstrated to inhibit the AR-mediated growth of prostate cancer. This protein also interacts with class III ubiquitin-conjugating enzymes (E2s) and may act as a ubiquitin-ligase (E3) in the ubiquitination of certain nuclear proteins. Five alternatively spliced transcript variants encoding two distinct isoforms have been reported. [provided by RefSeq
Other Designations
androgen receptor associated protein 54|triad2 protein
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Interactome
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