LIPG monoclonal antibody (M01), clone 4A9
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of LIPG expression in transfected 293T cell line by LIPG monoclonal antibody (M01), clone 4A9.
Lane 1: LIPG transfected lysate (Predicted MW: 56.8 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of LIPG transfected lysate using anti-LIPG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPG MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LIPG is 3 ng/ml as a capture antibody.
Western Blot detection against Immunogen (78.54 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full-length recombinant LIPG.
Immunogen
LIPG (AAH60825.1, 21 a.a. ~ 500 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SPVPFGPEGRLEDKLHKPKATQTEVKPSVRFNLRTSKDPEHEGCYLSVGHSQPLEDCSFNMTAKTFFIIHGWTMSGIFENWLHKLVSALHTREKDANVVVVDWLPLAHQLYTDAVNNTRVVGHSIARMLDWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGQELWFRKCRDGWRMKNETSPTVELP
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (80); Rat (79)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (78.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of LIPG expression in transfected 293T cell line by LIPG monoclonal antibody (M01), clone 4A9.
Lane 1: LIPG transfected lysate (Predicted MW: 56.8 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of LIPG transfected lysate using anti-LIPG monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LIPG MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged LIPG is 3 ng/ml as a capture antibody.ELISA
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Gene Info — LIPG
Entrez GeneID
9388GeneBank Accession#
BC060825Protein Accession#
AAH60825.1Gene Name
LIPG
Gene Alias
EDL, EL, PRO719
Gene Description
lipase, endothelial
Omim ID
603684Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene has substantial phospholipase activity and may be involved in lipoprotein metabolism and vascular biology. This protein is designated a member of the TG lipase family by its sequence and characteristic lid region which provides substrate specificity for enzymes of the TG lipase family. [provided by RefSeq
Other Designations
endothelial cell-derived lipase|endothelial lipase|lipoprotein lipase H
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Interactome
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Pathway
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Disease
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