STK17A monoclonal antibody (M03), clone 4D12
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant STK17A.
Immunogen
STK17A (AAH47696, 301 a.a. ~ 413 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
LLVKKPEDRATAEECLKHPWLTQSSIQEPSFRMEKALEEANALQEGHSVPEINSDTDKSETEESIVTEELIVVTSYTLGQCRQSEKEKMEQKAISKRFKFEEPLLQEIPGEFI
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (38.54 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
STK17A monoclonal antibody (M03), clone 4D12 Western Blot analysis of STK17A expression in HepG2 ( Cat # L019V1 ).Western Blot (Transfected lysate)
Western Blot analysis of STK17A expression in transfected 293T cell line by STK17A monoclonal antibody (M03), clone 4D12.
Lane 1: STK17A transfected lysate(46.6 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to STK17A on formalin-fixed paraffin-embedded human colon cancer. [antibody concentration 1.2 ug/ml]Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged STK17A is approximately 0.1ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of STK17A over-expressed 293 cell line, cotransfected with STK17A Validated Chimera RNAi ( Cat # H00009263-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with STK17A monoclonal antibody (M03) clone 4D12 (Cat # H00009263-M03 ). GAPDH ( 36.1 kDa ) used as specificity and loading control. -
Gene Info — STK17A
Entrez GeneID
9263GeneBank Accession#
BC047696Protein Accession#
AAH47696Gene Name
STK17A
Gene Alias
DRAK1
Gene Description
serine/threonine kinase 17a
Omim ID
604726Gene Ontology
HyperlinkGene Summary
This gene is a member of the DAP kinase-related apoptosis-inducing protein kinase family and encodes an autophosphorylated nuclear protein with a protein kinase domain. The protein has apoptosis-inducing activity. [provided by RefSeq
Other Designations
DAP kinase-related apoptosis-inducing protein kinase 1|death-associated protein kinase-related 1|serine/threonine kinase 17a (apoptosis-inducing)
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Interactome
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Disease
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