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Last updated: 2014/12/14
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CLDN1 monoclonal antibody (M02), clone 2E2-H5

  • Catalog # : H00009076-M02
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  • Specification
  • Product Description:
  • Mouse monoclonal antibody raised against a full-length recombinant CLDN1.
  • Immunogen:
  • CLDN1 (AAH12471.1, 1 a.a. ~ 211 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
  • Sequence:
  • MANAGLQLLGFILAFLGWIGAIVSTALPQWRIYSYAGDNIVTAQAMYEGLWMSCVSQSTGQIQCKVFDSLLNLSSTLQATRALMVVGILLGVIAIFVATVGMKCMKCLEDDEVQKMRMAVIGGAIFLLAGLAILVATAWYGNRIVQEFYDPMTPVNARYEFGQALFTGWAAASLCLLGGALLCCSCPRKTTSYPTPRPYPKPAPSSGKDYV
  • Host:
  • Mouse
  • Reactivity:
  • Human
  • Isotype:
  • IgG2b Kappa
  • Quality Control Testing:
  • Antibody Reactive Against Recombinant Protein.

    QC Testing of H00009076-M02
    Western Blot detection against Immunogen (48.95 KDa) .
  • Storage Buffer:
  • In 1x PBS, pH 7.4
  • Storage Instruction:
  • Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • Western Blot analysis of CLDN1 expression in transfected 293T cell line by CLDN1 monoclonal antibody (M02), clone 2E2-H5.

    Lane 1: CLDN1 transfected lysate (Predicted MW: 22.7 KDa).
    Lane 2: Non-transfected lysate.
  • Protocol Download
  • Immunofluorescence
  • Immunofluorescence
  • Immunofluorescence of monoclonal antibody to CLDN1 on Huh7 cell. [antibody concentration 10 ug/ml]
  • Protocol Download
  • Immunoprecipitation
  • Immunoprecipitation
  • Immunoprecipitation of CLDN1 transfected lysate using anti-CLDN1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with CLDN1 MaxPab rabbit polyclonal antibody.
  • Protocol Download
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Detection limit for recombinant GST tagged CLDN1 is 0.3 ng/ml as a capture antibody.
  • Protocol Download
  • ELISA
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • Huh7 cells were stained with CLDN1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
  • Application Image
  • Western Blot (Transfected lysate)
  • Western Blot (Transfected lysate)
  • enlarge
  • Western Blot (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • Sandwich ELISA (Recombinant protein)
  • enlarge
  • ELISA
  • Immunofluorescence (Circulating Tumor Cell)
  • Immunofluorescence (Circulating Tumor Cell)
  • enlarge
  • Gene Information
  • Entrez GeneID:
  • 9076
  • Gene Name:
  • CLDN1
  • Gene Alias:
  • CLD1,ILVASC,SEMP1
  • Gene Description:
  • claudin 1
  • Gene Summary:
  • Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome. [provided by RefSeq
  • Other Designations:
  • senescence-associated epithelial membrane protein 1
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