TOP3B 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
TOP3B transfected lysate.
Western Blot
Lane 1: TOP3B transfected lysate ( 96.70 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-TOP3B full-length
Host
Human
Theoretical MW (kDa)
96.7
Interspecies Antigen Sequence
Mouse (95); Rat (94)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-TOP3B antibody (H00008940-D01P) by Western Blots.
SDS-PAGE Gel
TOP3B transfected lysate.
Western Blot
Lane 1: TOP3B transfected lysate ( 96.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — TOP3B
Entrez GeneID
8940GeneBank Accession#
NM_003935Protein Accession#
NP_003926.1Gene Name
TOP3B
Gene Alias
FLJ39376
Gene Description
topoisomerase (DNA) III beta
Omim ID
603582Gene Ontology
HyperlinkGene Summary
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus relaxing the supercoils and altering the topology of DNA. The enzyme interacts with DNA helicase SGS1 and plays a role in DNA recombination, cellular aging and maintenance of genome stability. Alternative splicing of the C-terminus of this gene results in three transcript variants which have distinct tissue specificity; however, not all variants have been fully described. [provided by RefSeq
Other Designations
topoisomerase III beta
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Interactome
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Pathway
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