SMARCA5 MaxPab mouse polyclonal antibody (B01)
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Specification
Product Description
Mouse polyclonal antibody raised against a full-length human SMARCA5 protein.
Immunogen
SMARCA5 (AAH23144.1, 1 a.a. ~ 1052 a.a) full-length human protein.
Sequence
MSSAAEPPPPPPPESAPSKPAASIASGGSNSSNKGGPEGVAAQAVASAASAGPADAEMEEIFDDASPGKQKEIQEPDPTYEEKMQTDRANRFEYLLKQTELFAHFIQPAAQKTPTSPLKMKPGRPRIKKDEKQNLLSVGDYRHRRTEQEEDEELLTESSKATNVCTRFEDSPSYVKWGKLRDYQVRGLNWLISLYENGINGILADEMGLGKTLQTISLLGYMKHYRNIPGPHMVLVPKSTLHNWMSEFKRWVPTLRSVCLIGDKEQRAAFVRDVLLPGEWDVCVTSYEMLIKEKSVFKKFNWRYLVIDEAHRIKNEKSKLSEIVREFKTTNRLLLTGTPLQNNLHELWSLLNFLLPDVFNSADDFDSWFDTNNCLGDQKLVERLHMVLRPFLLRRIKADVEKSLPPKKEVKIYVGLSKMQREWYTRILMKDIDILNSAGKMDKMRLLNILMQLRKCCNHPYLFDGAEPGPPYTTDMHLVTNSGKMVVLDKLLPKLKEQGSRVLIFSQMTRVLDILEDYCMWRNYEYCRLDGQTPHDERQDSINAYNEPNSTKFVFMLSTRAGGLGINLATADVVILYDSDWNPQVDLQAMDRAHRIGQTKTVRVFRFITDNTVEERIVERAEMKLRLDSIVIQQGRLVDQNLNKIGKDEMLQMIRHGATHVFASKESEITDEDIDGILERGAKKTAEMNEKLSKMGESSLRNFTMDTESSVYNFEGEDYREKQKIAFTEWIEPPKRERKANYAVDAYFREALRVSEPKAPKAPRPPKQPNVQDFQFFPPRLFELLEKEILFYRKTIGYKVPRNPELPNAAQAQKEEQLKIDEAESLNDEELEEKEKLLTQGFTNWNKRDFNQFIKANEKWGRDDIENIAREVEGKTPEEVIEYSAVFWERCNELQDIEKIMAQIERGEARIQRRISIKKALDTKIGRYKAPFHQLRISYGTNKGKNYTEEEDRFLICMLHKLGFDKENVYDELRQCIRNSPQFRFDWFLKSRTAMELQRRCNTLITLIERENMELEEKEKAEKKKRGPKPSTQKRKMDGAPDGRGRKKKLKL
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (98); Rat (99)
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
No additive
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Note
For IHC and IF applications, antibody purification with Protein A will be needed prior to use.
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Applications
Western Blot (Tissue lysate)
SMARCA5 MaxPab polyclonal antibody. Western Blot analysis of SMARCA5 expression in human liver.Western Blot (Transfected lysate)
Western Blot analysis of SMARCA5 expression in transfected 293T cell line (H00008467-T01) by SMARCA5 MaxPab polyclonal antibody.
Lane 1: SMARCA5 transfected lysate(115.72 KDa).
Lane 2: Non-transfected lysate.
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to SMARCA5 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — SMARCA5
Entrez GeneID
8467GeneBank Accession#
BC023144.1Protein Accession#
AAH23144.1Gene Name
SMARCA5
Gene Alias
ISWI, SNF2H, WCRF135, hISWI, hSNF2H
Gene Description
SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 5
Omim ID
603375Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the SWI/SNF family of proteins. Members of this family have helicase and ATPase activities and are thought to regulate transcription of certain genes by altering the chromatin structure around those genes. The protein encoded by this gene is a component of the chromatin remodeling and spacing factor RSF, a facilitator of the transcription of class II genes by RNA polymerase II. The encoded protein is similar in sequence to the Drosophila ISWI chromatin remodeling protein. [provided by RefSeq
Other Designations
SWI/SNF-related matrix-associated actin-dependent regulator of chromatin a5|sucrose nonfermenting-like 5
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Interactome
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Publication Reference
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Posterior association networks and functional modules inferred from rich phenotypes of gene perturbations.
Wang X, Castro MA, Mulder KW, Markowetz F.
PLoS Computational Biology 2012 Jun; 8(6):e1002566.
Application:ChIP, Human, Human keratinocytes.
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Posterior association networks and functional modules inferred from rich phenotypes of gene perturbations.
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