CDK2AP1 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
CDK2AP1 transfected lysate.
Western Blot
Lane 1: CDK2AP1 transfected lysate ( 12.40 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-CDK2AP1 full-length
Host
Human
Theoretical MW (kDa)
12.4
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-CDK2AP1 antibody (H00008099-B02) by Western Blots.
SDS-PAGE Gel
CDK2AP1 transfected lysate.
Western Blot
Lane 1: CDK2AP1 transfected lysate ( 12.40 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — CDK2AP1
Entrez GeneID
8099GeneBank Accession#
NM_004642Protein Accession#
AAH34717.1Gene Name
CDK2AP1
Gene Alias
DOC1, DORC1, ST19, doc-1, p12DOC-1
Gene Description
cyclin-dependent kinase 2 associated protein 1
Omim ID
602198Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a specific CDK2-associated protein, which is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. This protein was found to also interact with DNA polymerase alpha/primase and mediate the phosphorylation of the large p180 subunit, which suggested the regulatory role in DNA replication during S phase of the cell cycle. A similar gene in hamster was isolated from, and functions as a growth suppressor of normal keratinocytes. [provided by RefSeq
Other Designations
CDK2-associated protein 1|CDK2-associated protein 1|Deleted in oral cancer-1|cyclin-dependent kinase 2-associated protein 1|putative oral cancer suppressor
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