WNT5A 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
WNT5A transfected lysate.
Western Blot
Lane 1: WNT5A transfected lysate ( 42.30 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-WNT5A full-length
Host
Human
Theoretical MW (kDa)
42.3
Interspecies Antigen Sequence
Mouse (99)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-WNT5A antibody (H00007474-D01P) by Western Blots.
SDS-PAGE Gel
WNT5A transfected lysate.
Western Blot
Lane 1: WNT5A transfected lysate ( 42.30 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — WNT5A
Entrez GeneID
7474GeneBank Accession#
NM_003392Protein Accession#
NP_003383.2Gene Name
WNT5A
Gene Alias
hWNT5A
Gene Description
wingless-type MMTV integration site family, member 5A
Omim ID
164975Gene Ontology
HyperlinkGene Summary
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 98%, 98% and 87% amino acid identity to the mouse, rat and the xenopus Wnt5A protein, respectively. The experiments performed in Xenopus laevis embryos identified that human frizzled-5 (hFz5) is the receptor for the Wnt5A ligand and the Wnt5A/hFz5 signaling mediates axis induction. [provided by RefSeq
Other Designations
WNT-5A protein
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Interactome
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Pathway
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Disease
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