VIM 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-VIM full-length
Host
Human
Theoretical MW (kDa)
53.7
Interspecies Antigen Sequence
Mouse (97); Rat (97)
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-VIM antibody (H00007431-D01P) by Western Blots.
SDS-PAGE Gel
VIM transfected lysate.
Western Blot
Lane 1: VIM transfected lysate ( 53.70 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — VIM
Entrez GeneID
7431GeneBank Accession#
NM_003380.2Protein Accession#
NP_003371.2Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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