VIM monoclonal antibody (M01), clone 3E9
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More Files
- More Functions
Western Blot (Cell lysate)
VIM monoclonal antibody (M01), clone 3E9 Western Blot analysis of VIM expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody (M01), clone 3E9.
Lane 1: VIM transfected lysate(53.7 KDa).
Lane 2: Non-transfected lysate.
Immunoprecipitation
Immunoprecipitation of VIM transfected lysate using anti-VIM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with VIM MaxPab rabbit polyclonal antibody.
Immunofluorescence (Circulating Tumor Cell)
A-549 cells were stained with VIM-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).
Immunofluorescence
Immunofluorescence of monoclonal antibody to VIM on HeLa cell. [antibody concentration 40 ug/ml]
Immunofluorescence
Immunofluorescence of monoclonal antibody to VIM on U-2 OS cell. [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (36.74 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant VIM.
Immunogen
VIM (AAH30573, 41 a.a. ~ 140 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
GSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRTNEKVELQELNDRFANYIDKVRFLEQQNKILLAELEQLKG
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (97); Rat (96)
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (36.74 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
VIM monoclonal antibody (M01), clone 3E9 Western Blot analysis of VIM expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of VIM expression in transfected 293T cell line by VIM monoclonal antibody (M01), clone 3E9.
Lane 1: VIM transfected lysate(53.7 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunoprecipitation
Immunoprecipitation of VIM transfected lysate using anti-VIM monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with VIM MaxPab rabbit polyclonal antibody.ELISA
Immunofluorescence (Circulating Tumor Cell)
A-549 cells were stained with VIM-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).Immunofluorescence
Immunofluorescence of monoclonal antibody to VIM on HeLa cell. [antibody concentration 40 ug/ml]Immunofluorescence
Immunofluorescence of monoclonal antibody to VIM on U-2 OS cell. [antibody concentration 10 ug/ml] -
Gene Info — VIM
Entrez GeneID
7431GeneBank Accession#
BC030573Protein Accession#
AAH30573Gene Name
VIM
Gene Alias
FLJ36605
Gene Description
vimentin
Omim ID
193060Gene Ontology
HyperlinkGene Summary
This gene encodes a member of the intermediate filament family. Intermediate filamentents, along with microtubules and actin microfilaments, make up the cytoskeleton. The protein encoded by this gene is responsible for maintaining cell shape, integrity of the cytoplasm, and stabilizing cytoskeletal interactions. It is also involved in the immune response, and controls the transport of low-density lipoprotein (LDL)-derived cholesterol from a lysosome to the site of esterification. It functions as an organizer of a number of critical proteins involved in attachment, migration, and cell signaling. Mutations in this gene causes a dominant, pulverulent cataract
Other Designations
OTTHUMP00000019224
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Interactome
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Disease
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