This antibody pair set comes with matched antibody pair to detect and quantify protein level of human TGFB1.
Reactivity:
Human
Quality Control Testing:
Standard curve using recombinant protein ( H00007040-P01 ) as an analyte.
Sandwich ELISA detection sensitivity ranging from 3 ng/ml to 100 ng/ml.
Supplied Product:
Antibody pair set content: 1. Capture antibody: mouse monoclonal anti-TGFB1 (100 ug) 2. Detection antibody: biotinylated mouse monoclonal anti-TGFB1, IgG2b Kappa (50 ug) *Reagents are sufficient for at least 3-5 x 96 well plates using recommended protocols.
Storage Instruction:
Store reagents of the antibody pair set at -20°C or lower. Please aliquot to avoid repeated freeze thaw cycle. Reagents should be returned to -20°C storage immediately after use.
Standard curve using TGFB1 293T overexpression lysate (non-denatured) as an analyte. Sandwich ELISA detection sensitivity ranging from approximately 243x to 3x dilution of the TGFB1 293T overexpression lysate (non-denatured).
TGFB is a multifunctional peptide that controls proliferation, differentiation, and other functions in many cell types. TGFB acts synergistically with TGFA (MIM 190170) in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGFB activation and signaling may result in apoptosis. Many cells synthesize TGFB and almost all of them have specific receptors for this peptide. TGFB1, TGFB2 (MIM 190220), and TGFB3 (MIM 190230) all function through the same receptor signaling systems.[supplied by OMIM