STK3 purified MaxPab rabbit polyclonal antibody (D01P)
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More Files
- More Functions
Western Blot (Cell lysate)
STK3 MaxPab rabbit polyclonal antibody. Western Blot analysis of STK3 expression in A-431.
Western Blot (Transfected lysate)
Western Blot analysis of STK3 expression in transfected 293T cell line (H00006788-T03) by STK3 MaxPab polyclonal antibody.
Lane 1: STK3 transfected lysate(56.30 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between STK3 and CASP3. HeLa cells were stained with anti-STK3 rabbit purified polyclonal 1:1200 and anti-CASP3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
Immunofluorescence
Immunofluorescence of purified MaxPab antibody to STK3 on HeLa cell. [antibody concentration 10 ug/ml]
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Specification
Product Description
Rabbit polyclonal antibody raised against a full-length human STK3 protein.
Immunogen
STK3 (NP_006272.2, 1 a.a. ~ 491 a.a) full-length human protein.
Sequence
MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF
Host
Rabbit
Reactivity
Human
Quality Control Testing
Antibody reactive against mammalian transfected lysate.
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
STK3 MaxPab rabbit polyclonal antibody. Western Blot analysis of STK3 expression in A-431.Western Blot (Transfected lysate)
Western Blot analysis of STK3 expression in transfected 293T cell line (H00006788-T03) by STK3 MaxPab polyclonal antibody.
Lane 1: STK3 transfected lysate(56.30 KDa).
Lane 2: Non-transfected lysate.
In situ Proximity Ligation Assay (Cell)
Proximity Ligation Analysis of protein-protein interactions between STK3 and CASP3. HeLa cells were stained with anti-STK3 rabbit purified polyclonal 1:1200 and anti-CASP3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).Immunofluorescence
Immunofluorescence of purified MaxPab antibody to STK3 on HeLa cell. [antibody concentration 10 ug/ml] -
Gene Info — STK3
Entrez GeneID
6788GeneBank Accession#
NM_006281.2Protein Accession#
NP_006272.2Gene Name
STK3
Gene Alias
FLJ90748, KRS1, MST2
Gene Description
serine/threonine kinase 3 (STE20 homolog, yeast)
Omim ID
605030Gene Ontology
HyperlinkGene Summary
Protein kinase activation is a frequent response of cells to treatment with growth factors, chemicals, heat shock, or apoptosis-inducing agents. This protein kinase activation presumably allows cells to resist unfavorable environmental conditions. The yeast 'sterile 20' (Ste20) kinase acts upstream of the mitogen-activated protein kinase (MAPK) cascade that is activated under a variety of stress conditions. MST2 was identified as a kinase that is activated by the proapoptotic agents straurosporine and FAS ligand (MIM 134638) (Taylor et al., 1996 [PubMed 8816758]; Lee et al., 2001 [PubMed 11278283]).[supplied by OMIM
Other Designations
serine/threonine kinase 3 (Ste20, yeast homolog)
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Interactome
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Pathway
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