CCL3 monoclonal antibody (M01), clone 4E7
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant CCL3.
Immunogen
CCL3 (NP_002974, 24 a.a. ~ 92 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
SLAADTPTACCFSYTSRQIPQNFIADYFETSSQCSKPGVIFLTKRSRQVCADPSEEWVQKYVSDLELSA
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (33.33 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of CCL3 expression in transfected 293T cell line by CCL3 monoclonal antibody (M01), clone 4E7.
Lane 1: CCL3 transfected lysate(10.1 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged CCL3 is approximately 0.1ng/ml as a capture antibody.ELISA
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Gene Info — CCL3
Entrez GeneID
6348GeneBank Accession#
NM_002983Protein Accession#
NP_002974Gene Name
CCL3
Gene Alias
G0S19-1, LD78ALPHA, MIP-1-alpha, MIP1A, SCYA3
Gene Description
chemokine (C-C motif) ligand 3
Gene Ontology
HyperlinkGene Summary
Macrophage inflammatory protein-1 is a so-called monokine that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes (Wolpe et al., 1988 [PubMed 3279154]). Sherry et al. (1988) [PubMed 3058856] demonstrated 2 protein components of MIP1, called by them alpha and beta.[supplied by OMIM
Other Designations
LD78 alpha beta|small inducible cytokine A3 (homologous to mouse Mip-1a)
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Interactome
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Pathway
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Disease
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Publication Reference
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A multiplex assay to measure RNA transcripts of prostate cancer in urine.
Quek SI, Ho ME, Loprieno MA, Ellis WJ, Elliott N, Liu AY.
PLoS One 2012 Sep; 7(9):e45656.
Application:IHC-Fr, Human, Human prostate cancer.
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A multiplex assay to measure RNA transcripts of prostate cancer in urine.
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