S100A8 293T Cell Transient Overexpression Lysate(Denatured)
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More Files
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SDS-PAGE Gel
S100A8 transfected lysate.
Western Blot
Lane 1: S100A8 transfected lysate ( 10.80 KDa)
Lane 2: Non-transfected lysate.
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Specification
Transfected Cell Line
293T
Plasmid
pCMV-S100A8 full-length
Host
Human
Theoretical MW (kDa)
10.8
Quality Control Testing
Transient overexpression cell lysate was tested with Anti-S100A8 antibody (H00006279-B02) by Western Blots.
SDS-PAGE Gel
S100A8 transfected lysate.
Western Blot
Lane 1: S100A8 transfected lysate ( 10.80 KDa)
Lane 2: Non-transfected lysate.Storage Buffer
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot
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Gene Info — S100A8
Entrez GeneID
6279GeneBank Accession#
NM_002964.3Protein Accession#
NP_002955.2Gene Name
S100A8
Gene Alias
60B8AG, CAGA, CFAG, CGLA, CP-10, L1Ag, MA387, MIF, MRP8, NIF, P8
Gene Description
S100 calcium binding protein A8
Omim ID
123885Gene Ontology
HyperlinkGene Summary
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq
Other Designations
OTTHUMP00000015329|OTTHUMP00000015330|S100 calcium-binding protein A8|S100 calcium-binding protein A8 (calgranulin A)|calgranulin A|cystic fibrosis antigen
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Interactome
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Disease
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