Transient overexpression cell lysate was tested with Anti-S100A8 antibody (H00006279-D01P) by Western Blots. SDS-PAGE Gel S100A8 transfected lysate. Western Blot Lane 1: S100A8 transfected lysate ( 10.34 KDa) Lane 2: Non-transfected lysate.
Storage Buffer:
1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
Storage Instruction:
Store at -80°C. Aliquot to avoid repeated freezing and thawing.
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in the inhibition of casein kinase and as a cytokine. Altered expression of this protein is associated with the disease cystic fibrosis. [provided by RefSeq
Other Designations:
OTTHUMP00000015329,OTTHUMP00000015330,S100 calcium-binding protein A8,S100 calcium-binding protein A8 (calgranulin A),calgranulin A,cystic fibrosis antigen