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RAF1 293T Cell Transient Overexpression Lysate(Denatured)

  • Catalog # : H00005894-T01
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  • Specification
  • Transfected Cell Line:
  • 293T
  • Plasmid:
  • pCMV-RAF1 full-length
  • Host:
  • Human
  • Theoretical MW (kDa):
  • 71.39
  • Quality Control Testing:
  • Transient overexpression cell lysate was tested with Anti-RAF1 antibody (H00005894-B01) by Western Blots.
    Western Blot
    QC Testing of H00005894-T01
    Lane 1: RAF1 transfected lysate ( 73.1 KDa)
    Lane 2: Non-transfected lysate.
    SDS-PAGE Gel
    QC Testing of H00005894-T01
    RAF1 transfected lysate.
  • Storage Buffer:
  • 1X Sample Buffer (50 mM Tris-HCl, 2% SDS, 10% glycerol, 300 mM 2-mercaptoethanol, 0.01% Bromophenol blue)
  • Storage Instruction:
  • Store at -80°C. Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Western Blot
  • Application Image
  • Western Blot
  • Gene Information
  • Entrez GeneID:
  • 5894
  • Protein Accession#:
  • -
  • Gene Name:
  • RAF1
  • Gene Alias:
  • CRAF,NS5,Raf-1,c-Raf
  • Gene Description:
  • v-raf-1 murine leukemia viral oncogene homolog 1
  • Gene Summary:
  • This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2. [provided by RefSeq
  • Other Designations:
  • Oncogene RAF1,raf proto-oncogene serine/threonine protein kinase
  • Interactome
  • Interactome
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