PTK9 monoclonal antibody (M05), clone 1D12
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More Files
- More Functions
Western Blot (Transfected lysate)
Western Blot analysis of PTK9 expression in transfected 293T cell line by PTK9 monoclonal antibody (M05), clone 1D12.
Lane 1: PTK9 transfected lysate(43.9 KDa).
Lane 2: Non-transfected lysate.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PTK9 is approximately 0.03ng/ml as a capture antibody.
RNAi Knockdown (Antibody validated)
Western blot analysis of PTK9 over-expressed 293 cell line, cotransfected with PTK9 Validated Chimera RNAi ( Cat # H00005756-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTK9 monoclonal antibody (M05) clone 1D12 (Cat # H00005756-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to TWF1 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot detection against Immunogen (44.66 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a partial recombinant PTK9.
Immunogen
PTK9 (AAH22344, 80 a.a. ~ 252 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
VAFPISREAFQALEKLNNRQLNYVQLEIDIKNEIIILANTTNTELKDLPKRIPKDSARYHFFLYKHSHEGDYLESIVFIYSMPGYTCSIRERMLYSSCKSRLLEIVERQLQMDVIRKIEIDNGDELTADFLYEEVHPKQHAHKQSSAKPKGPAGKRGIRRLIRGPAETEATTD
Host
Mouse
Reactivity
Human
Isotype
IgG2a Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (44.66 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Transfected lysate)
Western Blot analysis of PTK9 expression in transfected 293T cell line by PTK9 monoclonal antibody (M05), clone 1D12.
Lane 1: PTK9 transfected lysate(43.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PTK9 is approximately 0.03ng/ml as a capture antibody.ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PTK9 over-expressed 293 cell line, cotransfected with PTK9 Validated Chimera RNAi ( Cat # H00005756-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PTK9 monoclonal antibody (M05) clone 1D12 (Cat # H00005756-M05 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to TWF1 on HeLa cell . [antibody concentration 10 ug/ml] -
Gene Info — TWF1
Entrez GeneID
5756GeneBank Accession#
BC022344Protein Accession#
AAH22344Gene Name
TWF1
Gene Alias
A6, MGC23788, MGC41876, PTK9
Gene Description
twinfilin, actin-binding protein, homolog 1 (Drosophila)
Omim ID
610932Gene Ontology
HyperlinkGene Summary
This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq
Other Designations
A6 protein tyrosine kinase|PTK9 protein tyrosine kinase 9|protein tyrosine kinase 9|twinfilin 1
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Interactome
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Disease
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