PSMA7 monoclonal antibody (M01), clone 1A10-3G12
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More Files
- More Functions
Western Blot (Tissue lysate)
PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.
Western Blot (Cell lysate)
PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Western Blot analysis of PSMA7 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.
Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).
Lane 2: Non-transfected lysate.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5 ug/ml]
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 5 ug/ml]
Immunoprecipitation
Immunoprecipitation of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.
Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10 ug/ml]
Western Blot detection against Immunogen (53.02 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PSMA7.
Immunogen
PSMA7 (AAH04427, 1 a.a. ~ 248 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MSYDRAITVFSPDGHLFQVEYAQEAVKKGSTAVGVRGRDIVVLGVEKKSVAKLQDERTVRKICALDDNVCMAFAGLTADARIVINRARVECQSHRLTVEDPVTVEYITRYIASLKQRYTQSNGRRPFGISALIVGFDFDGTPRLYQTDPSGTYHAWKANAIGRGAKSVREFLEKNYTDEAIETDDLTIKLVIKALLEVVQSGGKNIELAVMRRDQSLKILNPEEIEKYVAEIEKEKEENEKKKQKKAS
Host
Mouse
Reactivity
Human
Interspecies Antigen Sequence
Mouse (99); Rat (99)
Isotype
IgG2b Kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (53.02 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Tissue lysate)
PSMA7 monoclonal antibody (M01), clone 1A10-3G12. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.Western Blot (Cell lysate)
PSMA7 monoclonal antibody (M01), clone 1A10-3G12 Western Blot analysis of PSMA7 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody (M01), clone 1A10-3G12.
Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5 ug/ml]Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosquamous cell carcinoma. [antibody concentration 5 ug/ml]Immunoprecipitation
Immunoprecipitation of PSMA7 transfected lysate using anti-PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 MaxPab rabbit polyclonal antibody.Sandwich ELISA (Recombinant protein)
Detection limit for recombinant GST tagged PSMA7 is approximately 1ng/ml as a capture antibody.ELISA
Immunofluorescence
Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10 ug/ml] -
Gene Info — PSMA7
Entrez GeneID
5688GeneBank Accession#
BC004427Protein Accession#
AAH04427Gene Name
PSMA7
Gene Alias
C6, HSPC, MGC3755, RC6-1, XAPC7
Gene Description
proteasome (prosome, macropain) subunit, alpha type, 7
Omim ID
606607Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. This particular subunit has been shown to interact specifically with the hepatitis B virus X protein, a protein critical to viral replication. In addition, this subunit is involved in regulating hepatitis virus C internal ribosome entry site (IRES) activity, an activity essential for viral replication. This core alpha subunit is also involved in regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. Multiple isoforms of this subunit arising from alternative splicing may exist but alternative transcripts for only two isoforms have been defined. A pseudogene has been identified on chromosome 9. [provided by RefSeq
Other Designations
OTTHUMP00000031449|proteasome alpha 7 subunit|proteasome subunit RC6-1|proteasome subunit XAPC7|proteasome subunit alpha 4
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Interactome
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Pathway
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Disease
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Publication Reference
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PSMA7 inhibits the tumorigenicity of A549 human lung adenocarcinoma cells.
Tan JY, Huang X, Luo YL.
Molecular and Cellular Biochemistry 2012 Jul; 366(1-2):131.
Application：WB-Ce, Human, A-549 cells.
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Proteomic identification of putative biomarkers of radiotherapy resistance: a possible role for the 26S proteasome?
Smith L, Qutob O, Watson MB, Beavis AW, Potts D, Welham KJ, Garimella V, Lind MJ, Drew PJ, Cawkwell L.
Neoplasia 2009 Nov; 11(11):1194.
Application：WB-Ce, Human, MCF-7, MDA-MB-231, T-47D cells.
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PSMA7 inhibits the tumorigenicity of A549 human lung adenocarcinoma cells.
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