PSMA1 monoclonal antibody (M01), clone 1D9-1C7
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More Files
- More Functions
Western Blot (Cell lysate)
PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Western Blot analysis of PSMA1 expression in HeLa ( Cat # L013V1 ).
Western Blot (Transfected lysate)
Western Blot analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7.
Lane 1: PSMA1 transfected lysate(30.2 KDa).
Lane 2: Non-transfected lysate.
RNAi Knockdown (Antibody validated)
Western blot analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi ( Cat # H00005682-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody (M01), clone 1D9-1C7 (Cat # H00005682-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Immunofluorescence
Immunofluorescence of monoclonal antibody to PSMA1 on HeLa cell. [antibody concentration 60 ug/ml]
Western Blot detection against Immunogen (54.67 KDa) .
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Specification
Product Description
Mouse monoclonal antibody raised against a full length recombinant PSMA1.
Immunogen
PSMA1 (AAH02577, 1 a.a. ~ 263 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Sequence
MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQKKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPTQRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFMECNLNELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLEGLEERPQRKAQPAQPADEPAEKADEPMEH
Host
Mouse
Reactivity
Human
Isotype
IgG1 kappa
Quality Control Testing
Antibody Reactive Against Recombinant Protein.
Western Blot detection against Immunogen (54.67 KDa) .
Storage Buffer
In 1x PBS, pH 7.4
Storage Instruction
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Applications
Western Blot (Cell lysate)
PSMA1 monoclonal antibody (M01), clone 1D9-1C7 Western Blot analysis of PSMA1 expression in HeLa ( Cat # L013V1 ).Western Blot (Transfected lysate)
Western Blot analysis of PSMA1 expression in transfected 293T cell line by PSMA1 monoclonal antibody (M01), clone 1D9-1C7.
Lane 1: PSMA1 transfected lysate(30.2 KDa).
Lane 2: Non-transfected lysate.
Western Blot (Recombinant protein)
ELISA
RNAi Knockdown (Antibody validated)
Western blot analysis of PSMA1 over-expressed 293 cell line, cotransfected with PSMA1 Validated Chimera RNAi ( Cat # H00005682-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with PSMA1 monoclonal antibody (M01), clone 1D9-1C7 (Cat # H00005682-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.Immunofluorescence
Immunofluorescence of monoclonal antibody to PSMA1 on HeLa cell. [antibody concentration 60 ug/ml] -
Gene Info — PSMA1
Entrez GeneID
5682GeneBank Accession#
BC002577Protein Accession#
AAH02577Gene Name
PSMA1
Gene Alias
HC2, MGC14542, MGC14575, MGC14751, MGC1667, MGC21459, MGC22853, MGC23915, NU, PROS30
Gene Description
proteasome (prosome, macropain) subunit, alpha type, 1
Omim ID
602854Gene Ontology
HyperlinkGene Summary
The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the peptidase T1A family, that is a 20S core alpha subunit. Alternative splicing results in multiple transcript variants encoding distinct isoforms
Other Designations
30 kDa prosomal protein|macropain subunit nu|multicatalytic endopeptidase complex subunit C2|proteasome alpha 1 subunit|proteasome component C2|proteasome nu chain|proteasome subunit nu|proteasome subunit, alpha-type, 1|protein P30-33K
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Interactome
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Pathway
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Publication Reference
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The Role of Translationally Controlled Tumor Protein in Tumor Growth and Metastasis of Colon Adenocarcinoma Cells.
Ma Q, Geng Y, Xu W, Wu Y, He F, Shu W, Huang M, Du H, Li M.
Journal of Proteome Research 2010 Jan; 9(1):40.
Application：WB-Tr, Human, LoVo, SW620 cells.
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The Role of Translationally Controlled Tumor Protein in Tumor Growth and Metastasis of Colon Adenocarcinoma Cells.
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